A microtome is a specialised precision cutting instrument that slices sections from a block of embedded tissue accurately and repeatedly. Different types of microtomes, as well as specialised microtomes, are used to section paraffin and plastic-embedded tissues and frozen tissues. A sharp knife and a tissue block are held in a fixed relationship in any Microtomes. Each pass of the tissue through the knife advances the tissue block by a predetermined amount—the section thickness. The thickness of frozen sections is typically 8 to 15 m, wax sections 4-10 m, and plastic histological sections 0.5-3 m. Sections for electron microscopy must be extremely thin, about 200 times thinner than wax sections. Typically plastic sections used in transmission electron microscopy (TEM) are cut in the range 60–100 nm.
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